umuc 14  (CancerTools Org)

Journal: International Journal of Molecular Sciences

Article Title: BGJ398, A Pan-FGFR Inhibitor, Overcomes Paclitaxel Resistance in Urothelial Carcinoma with FGFR1 Overexpression

doi: 10.3390/ijms19103164

Figure Lengend Snippet: FGFR1 expression is correlated with EMT features and PTX resistance in UC cell lines. ( A ) T24, J82, RT4, UMUC-14, HTB5, and HTB9 cells were evaluated basal expression of FGFRs and EMT-associated proteins by Western blotting; β-actin served as a loading control. ( B ) Colony formation assay. T24, J82, RT4, and UMUC-14 cells were grown for 7 days, then stained with Coomassie Brilliant Blue and counted. ( C ) T24, J82, RT4, and UMUC-14 cells were treated with 0, 1, 10, 100, and 1000 nM PTX for 3 days. IC 50 values were calculated using CalcuSyn (BioSoft, Ferguson, MO, USA). Data represent the mean ± standard deviation of five replicates. ( D ) Cell cycle analysis by propidium iodide staining and flow cytometry. A total of 1 × 10 6 cells were seeded in 60-mm plates and treated with 0, 5, and 10 nM PTX for 48 h. Data are presented as histograms (blue, G0/G1 phase; green, S phase, and red, G2/M phase). ( E ) Cyclin B , p21 , p27 , γ-H2AX , caspase-3 , and PARP expression in T24, J82, UMUC-14, and RT4 cells, as determined by Western blotting; β-actin served as the loading control.

Article Snippet: UMUC-14, RT4, T24, J82, HTB5, and HTB9 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Western Blot, Control, Colony Assay, Staining, Standard Deviation, Cell Cycle Assay, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: BGJ398, A Pan-FGFR Inhibitor, Overcomes Paclitaxel Resistance in Urothelial Carcinoma with FGFR1 Overexpression

doi: 10.3390/ijms19103164

Figure Lengend Snippet: FGFR inhibition enhances PTX-induced cell cycle arrest in mesenchymal-type UC cell lines. ( A ) T24, J82, RT4, and UMUC-14 cells were treated with 0, 0.1, 1, and 10 μM BJG398 for 3 days. IC 50 values were calculated using CalcuSyn (BioSoft, Ferguson, MO, USA). Data represent mean ± standard deviation of five replicates. ( B ) Cells were exposed to increasing concentrations of PTX and BGJ398 combinations at a fixed ratio. Viable cells were assayed and CI was calculated using CalcuSyn software. Lines above each bar represent CI for that dose combination ( n = 3). ( C ) Cell cycle distribution and apoptosis were analyzed by propidium iodide staining and flow cytometry. A total of 1 × 10 6 cells were seeded in a 60-mm plate and treated with indicated concentrations of PTX and BGJ398 for 48 h ( n = 3). ( D ) Quantification of cell cycle distribution of T24, J82, RT4, and UMUC-14 cells.

Article Snippet: UMUC-14, RT4, T24, J82, HTB5, and HTB9 cells were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Inhibition, Standard Deviation, Software, Staining, Flow Cytometry